Journal: BMC Research Notes

Article Title: Construction and use of a prokaryotic expression system for Helicobacter pylori AhpC

doi: 10.1186/1756-0500-5-328

Figure Lengend Snippet: Strains and plasmid used in the study

Article Snippet: DH10B , Cloning strain , Promega.

Techniques: Plasmid Preparation, Cloning

Journal: PLoS ONE

Article Title: Whole Methylome Analysis by Ultra-Deep Sequencing Using Two-Base Encoding

doi: 10.1371/journal.pone.0009320

Figure Lengend Snippet: Plots A ( bis-sol ) and B ( bis gel ) show the coverage of sequencing reads against the +/− bisulfite converted references (dark/light blue) and the normal reference (red). As a comparison, plot C shows the coverage of a regular non-bisulfited DH10B sequencing run matched towards the normal reference (yellow) (Data from SOLiD software communtiy web-site). The missing coverage in all three plots corresponds to a 133 kb perfect repeat in tandem. Since reads will match to both repeats, they will not show up under the unique matches.

Article Snippet: Bacterial DH10B gDNA (Lofstrand Labs Limited, Gaithersburg, MD, USA) (5 µg) was sheared into fragments in a 13×65 mm borosilicate tube (Covaris, Woburn, MA, USA) in 500 µL 10 mM Tris, pH 8.0, plus 5% w/v 2-micron borosilicate glass dry spheres (Duke Scientific Corporation, Fremont, CA, USA), using a Covaris S2 (Covaris, Woburn, MA, USA) (shearing conditions: cycle no. 10, bath temperature 5°C, Mode: power tracking, duty cycle 20%, intensity 10, cycles/burst 1000, time 60 sec).

Techniques: Sequencing, Software

Journal: Microbial Cell Factories

Article Title: High production of ectoine from methane in genetically engineered Methylomicrobium alcaliphilum 20Z by preventing ectoine degradation

doi: 10.1186/s12934-024-02404-2

Figure Lengend Snippet: Strains and vectors used in this study

Article Snippet: Escherichia coli DH10B , F- mcrA ∆( mrr-hsdRMS-mcrBC ) φ80 lac Z∆M15 ∆ lac X74 rec A1 end A1 ara ∆139 ∆( ara, leu )7697 gal U gal K λ- rps L (StrR) nup G , RBC Bioscience.

Techniques: Plasmid Preparation